Plasmid_Backbone

Part:BBa_K3579017:Design

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF_bb1AB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal NheI site found at 6610
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal BglII site found at 409
    Illegal BglII site found at 491
    Illegal BamHI site found at 537
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
    Illegal NgoMIV site found at 6758
    Illegal AgeI site found at 6227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6877
    Illegal BsaI.rc site found at 6024
    Illegal SapI site found at 3135
    Illegal SapI site found at 3911


Design Notes

BsaI and BbsI restriction sites have been removed



Source

pRMC2 (Plasmid #68940)


References